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Publications

2024

Human NLRP3 inflammasome activation leads to formation of condensate at the microtubule organizing center

September 17, 2024 02:30 PM
bioRXiv doi.org/10.1101/2024.09.12.612739

Wang, J., Wu, M., Magupalli, V., Dahlberg, P., Wu, H., and Jensen, G.

2023

Bdellovibrio predation cycle characterized at nanometre-scale resolution with cryo-electron tomography

June 27, 2023 11:24 AM
Nature Microbiology /10.1038/s41564-023-01401-2

Kaplan, M., Chang, Y.-W., Oikonomou, C. M., Nicolas, W. J., Jewett, A. I., Kreida, S., Duta, P., Rettberg, L. A., Maggi, S., and Jensen, G. J.

Structure and Function of the Dot/Icm T4SS

April 05, 2023 12:45 PM
bioRxiv doi.org/10.1101/2023.03.22.533729

Dutka, P., Liu, Y., Maggi, Ghosal, D., Wang, J., Carter, S. D., Zhao, W., Vijayrajratnam, S., Vogel, J. P., and Jensen, G. J.

Cryo-EM structure of the Agrobacterium tumefaciens T4SS-associated T-pilus reveals stoichiometric protein-phospholipid assembly

April 05, 2023 12:40 PM
Structure S0969-2126(23)00039 -4

Kreida, S., Narita, A., Johnson, M. D., Tocheva, E. I., Das, A., Ghosal, D., and Jensen, G. J.

2022

A cryogenic, coincident fluorescence, electron, and ion beam microscope

March 16, 2023 01:57 PM
eLife

Boltje DB, Hoogenboom JP, Jakobi AJ, Jensen GJ, Jonker CTH, Kaag MJ, Koster AJ, Last MGF, de Agrela Pinto C, Plitzko JM, Raunser S, Tacke S, Wang Z, van der Wee EB, Wepf R, den Hoedt S

Subtomogram averaging for biophysical analysis and supramolecular context

March 16, 2023 01:57 PM
Journal of Structural Biology: X

Metskas LA, Wilfong R, Jensen GJ

Cryo-EM structure of the Agrobacterium tumefaciens type IV secretion system-associated T-pilus reveals stoichiometric protein-phospholipid assembly

March 16, 2023 01:56 PM
bioRxiv

Kreida S*, Narita A*, Johnson MD, Tocheva EI, Das A, Ghosal D+, Jensen GJ+

Geometric effects in gas vesicle buckling under ultrasound

March 16, 2023 01:56 PM
Biophysical Journal

Salahshoor H*, Yao Y*, Dutka P*, Nyström NN, Jin Z, Min E, Malounda D, Jensen GJ, Ortiz M, Shapiro MG

Structural remodeling of Coxiella burnetii during its biphasic developmental cycle revealed by cryo-electron tomography

March 16, 2023 01:55 PM
bioRxiv

Kaplan M*, Shepherd DC*, Vankadari N, Kim KW, Larson CL, Dutka P, Beare PA, Krzymowski E, Heinzen RA, Jensen GJ+, Ghosal D+

Rubisco forms a lattice inside alpha-carboxysomes

March 16, 2023 01:54 PM
Nature Communications

Metskas LA, Ortega D, Oltrogge LM, Blikstad C, Lovejoy DR, Laughlin TG, Savage DF, Jensen GJ

Archaic chaperone–usher pili self-secrete into superelastic zigzag springs

March 16, 2023 01:53 PM
Nature

Pakharukova N*, Malmi H*, Tuittila M, Dahlberg T, Ghosal D, Chang Y-W, Myint SL, Paavilainen S, Knight SD, Lamminmäki U, Uhlin BE, Andersson M, Jensen G, Zavialov AV

Discovery of a Novel Inner Membrane-Associated Bacterial Structure Related to the Flagellar Type III Secretion System

March 16, 2023 01:53 PM
Journal of Bacteriology

Kaplan M, Oikonomou CM, Wood CR, Chreifi G, Ghosal D, Dobro MJ, Yao Q, Pal RR, Baidya AK, Liu Y, Maggi S, McDowall AW, Ben-Yehuda S, Rosenshine I, Briegel A, Beeby M, Chang Y-W, Shaffer CL, Jensen GJ

Structure of Anabaena flos-aquae gas vesicles revealed by cryo-ET

March 16, 2023 01:52 PM
bioRxiv

Dutka P, Metskas LA, Hurt RC, Salahshoor H, Wang T-Y, Malounda D, Lu G, Chou T-F, Shapiro MG+, Jensen GJ+

Highlighted in Nature Structural & Molecular Biology Vol. 30

Dynamic structural adaptations enable the endobiotic predation of Bdellovibrio bacteriovorus

March 16, 2023 01:51 PM
bioRxiv

Kaplan M*, Chang Y-W*, Oikonomou CM, Nicolas WJ, Jewett AI, Kreida S, Dutka P, Rettberg LA, Maggi S, Jensen GJ

Cryo-electron tomography of the onion cell wall shows bimodally oriented cellulose fibers and reticulated homogalacturonan networks

March 16, 2023 01:51 PM
Current Biology

Nicolas WJ, Faessler F, Dutka P, Schur FKM, Jensen GJ, Meyerowitz EM

Montage electron tomography of vitrified specimens

March 16, 2023 01:49 PM
Journal of Structural Biology

Peck A, Carter SD, Mai H, Chen S, Burt A, Jensen GJ

Novel transient cytoplasmic rings stabilize assembling bacterial flagellar motors

March 16, 2023 01:48 PM
EMBO Journal

Kaplan M, Oikonomou CM, Wood CR, Chreifi G, Subramanian P, Ortega DR, Chang Y-W, Beeby M, Shaffer CL, Jensen GJ

UVC inactivation of pathogenic samples suitable for cryo-EM analysis

March 16, 2023 01:46 PM
Communications Biology

Depelteau JS, Renault L, Althof N, Cassidy CK, Mendonça LM, Jensen GJ, Resch GP, Briegel A

2021

Nanoscale details of mitochondrial fission revealed by cryo-electron tomography

March 16, 2023 01:28 PM
bioRxiv

Mageswaran SK*, Grotjahn DA*+, Zeng X, Barad BA, Medina M, Hoang MH, Dobro MJ, Chang Y-W, Xu M, Yang WY, Jensen GJ+

A bacterial membrane sculpting protein with BAR domain-like activity

March 16, 2023 01:28 PM
eLife

Phillips DA*, Zacharoff LA*, Hampton CM, Chong GW, Malanoski AP, Metskas LA, Xu S, Bird LJ, Eddie BJ, Miklos AE, Jensen GJ, Drummy LF, El-Naggar MY, Glaven SM

The stress-sensing domain of activated IRE1α forms helical filaments in narrow ER membrane tubes

March 16, 2023 01:27 PM
Science

Tran N-H*, Carter SD*, De Maziére A, Ashkenazi A, Klumperman J, Walter P+, Jensen GJ+

The Atlas of Bacterial & Archaeal Cell Structure: an Interactive Open-Access Microbiology Textbook

March 16, 2023 01:27 PM
Journal of Microbiology & Biology Education

Oikonomou CM, Jensen GJ

In situ imaging of bacterial outer membrane projections and associated protein complexes using electron cryo-tomography

March 16, 2023 01:26 PM
eLife

Kaplan M, Chreifi G, Metskas LA, Liedtke J, Wood CR, Oikonomou CM, Nicolas WJ, Subramanian P, Zacharoff LA, Wang Y, Chang Y-W, Beeby M, Dobro M, Zhu Y, McBride MJ, Briegel A, Shaffer C, Jensen GJ

Loss of the Bacterial Flagellar Motor Switch Complex upon Cell Lysis

March 16, 2023 01:25 PM
mBio

Kaplan M, Tocheva EI, Briegel A, Dobro MJ, Chang Y-W, Subramanian P, McDowall AW, Beeby M, Jensen GJ

Generation of ordered protein assemblies using rigid three-body fusion

March 16, 2023 01:24 PM
PNAS

Vulovic I, Yao Q*, Park Y-J*, Courbet A, Norris A, Busch F, Sahasrabuddhe A, Merten H, Sahtoe DD, Ueda G, Fallas JA, Weaver SJ, Hsia Y, Langan RA, Plückthun A, Wysocki VH, Veesler D, Jensen GJ, Baker D

Challenges in solving structures from radiation-damaged tomograms of protein nanocrystals assessed by simulation

March 16, 2023 01:23 PM
Acta Crystallographica Section D

Peck A, Yao Q, Brewster AS, Zwart PH, Heumann JM, Sauter NK, Jensen GJ

Programmed Flagellar Ejection in Caulobacter crescentus Leaves PL-subcomplexes

March 16, 2023 01:23 PM
Journal of Molecular Biology

Kaplan M, Wang Y, Chreifi G, Zhang L, Chang Y-W, Jensen GJ

A cryo–electron tomography workflow reveals protrusion-mediated shedding on injured plasma membrane

March 16, 2023 01:22 PM
Science Advances

Mageswaran SK, Yang WY+, Chakrabarty Y, Oikonomou CM, Jensen GJ+

Rapid tilt-series method for cryo-electron tomography: Characterizing stage behavior during FISE acquisition

March 16, 2023 01:21 PM
Journal of Structural Biology

Chreifi G, Chen S, Jensen GJ

Measuring gas vesicle dimensions by electron microscopy

March 16, 2023 01:20 PM
Protein Science

Dutka P, Malounda D, Metskas LA, Chen S, Hurt RC, Lu GJ, Jensen GJ+, Shapiro MK+

Moltemplate: A Tool for Coarse-Grained Modeling of Complex Biological Matter and Soft Condensed Matter Physics

March 16, 2023 01:19 PM
Journal of Molecular Biology

Jewett AI+, Stelter D, Lambert J, Saladi SM, Roscioni OM, Ricci M, Autin L, Maritan M, Bashusqeh SM, Keyes T, Dame RT, Shea J-E, Jensen GJ, Goodsell DS+

2020

In Situ Imaging and Structure Determination of Biomolecular Complexes Using Electron Cryo-Tomography

March 16, 2023 01:18 PM
Methods in Molecular Biology: CryoEM

Kaplan M, Nicolas WJ, Zhao W, Carter SD, Metskas LA, Chreifi G, Ghosal D, Jensen GJ

Visualizing insulin vesicle neighborhoods in β cells by cryo–electron tomography

March 16, 2023 01:17 PM
Science Advances

Zhang X, Carter SD, Singla J, White KL, Butler PC, Stevens RC+, Jensen GJ+

Simulations of Proposed Mechanisms of FtsZ-Driven Cell Constriction

March 16, 2023 01:16 PM
Journal of Bacteriology

Nguyen LT, Oikonomou CM, Jensen GJ

Structure of the Bacterial Cellulose Ribbon and Its Assembly-Guiding Cytoskeleton by Electron Cryotomography

March 16, 2023 01:16 PM
Journal of Bacteriology

Nicolas WJ, Ghosal D, Tocheva EI, Meyerowitz EM, Jensen GJ

CryoEM structure of the type IVa pilus secretin required for natural competence in Vibrio cholerae

March 16, 2023 01:13 PM
Nature Communications

Weaver SJ, Ortega DR, Sazinsky MH, Dalia TN, Dalia AB, Jensen GJ

PilY1 and minor pilins form a complex priming the type IVa pilus in Myxococcus xanthus

March 16, 2023 01:11 PM
Nature Communications

Treuner-Lange A, Chang Y-W, Glatter T, Herfurth M, Lindow S, Chreifi G, Jensen GJ, Søgaard-Andersen L

Effects of antimicrobial photodynamic therapy on antibiotic-resistant Escherichia coli

March 16, 2023 01:10 PM
Photodiagnosis and Photodynamic Therapy

Garcez AS*+, Kaplan M*, Jensen GJ, Scheidt FR, Oliveira EM, Suzuki SS

In situ structure and organisation of the type IX secretion system

March 16, 2023 01:09 PM
bioRxiv

Gorasia DG*, Chreifi G*, Seers CA, Butler CA, Heath JE, Glew MD, McBride MJ, Subramanian P, Kjaer A, Jensen GJ+, Veith PD+, Reynolds EC+

Repurposing a chemosensory macromolecular machine

March 16, 2023 01:08 PM
Nature Communications

Ortega DR, Yang W, Subramanian P, Mann P, Kjær A, Chen S, Watts KJ, Pirbadian A, Collins DA, Kooger R, Kalyuzhnaya MG, Ringgaard S, Briegel A+, Jensen GJ+

Bacterial flagellar motor PL-ring disassembly subcomplexes are widespread and ancient

March 16, 2023 01:06 PM
PNAS

Kaplan M, Sweredoski MJ, Rodrigues JPGLM, Tocheva EI, Chang Y-W, Ortega DR, Beeby M, Jensen GJ

Ribosome-associated vesicles: A dynamic subcompartment of the endoplasmic reticulum in secretory cells

March 16, 2023 01:05 PM
Science Advances

Carter SD*, Hampton CM*, Langlois R*, Melero R, Farino ZJ, Calderon MJ, Li W, Wallace CT, Tran NH, Grassucci RA, Siegmund SE, Pemberton J, Morgenstern TJ, Eisenman L, Aguilar JI, Greenberg NL, Levy ES, Yi E, Mitchell WG, Rice WJ, Wigge C, Pilli J, George EW, Aslanoglou D, Courel M, Freyberg RJ, Javitch JA, Wills ZP, Area-Gomez E, Shiva S, Bartolini F, Volchuk A, Murray SA, Aridor M, Fish KN, Walter P, Balla T, Fass D, Wolf SG, Watkins SC, Carazo JM, Jensen GJ+, Frank J+, Freyberg Z+

2019

In vivo structure of the Legionella type II secretion system by electron cryotomography

March 16, 2023 12:47 PM
Nature Microbiology

Ghosal D, Kim KW, Zheng H, Kaplan M, Truchan HK, Lopez AE, McIntire IE, Vogel JP, Cianciotto NP, Jensen GJ

Bacterial swarming reduces Proteus mirabilis and Vibrio parahaemolyticus cell stiffness and increases β-lactam susceptibility

March 16, 2023 12:46 PM
mBio

Auer GK, Oliver PM, Rajendram M, Lin T-Y, Yao Q, Jensen GJ, Weibel DB

DDX-Interface: A factory of interoperable scientific gateways

March 16, 2023 12:45 PM
Gateways 2019 (Conference)

James A, Chacon R, Buddenhagen J, Ortega DR, Rees-Lee P, Jensen G, James DR

Distinct chemotaxis protein paralogs assemble into chemoreceptor signaling arrays to coordinate signaling output

March 16, 2023 12:44 PM
mBio

O’Neal L*, Gullett JM*, Aksenova A, Hubler A, Briegel A, Ortega D, Kjaer A, Jensen G, Alexandre G

Qualitative analyses of polishing and precoating FIB milled crystals for MicroED

March 16, 2023 12:34 PM
Structure

Martynowycz MW, Zhao W, Hattne J, Jensen GJ, Gonen T

In situ imaging of the bacterial flagellar motor disassembly and assembly processes

March 16, 2023 12:32 PM
EMBO Journal

Kaplan M, Subramanian P, Ghosal D, Oikonomou CM, Pirbadian S, Starwalt-Lee R, Mageswaran SK, Ortega DR, Gralnick JA, El-Naggar MY, Jensen GJ

Fusion of DARPin to aldolase enables visualization of small protein by cryoEM

March 16, 2023 12:31 PM
Structure

Yao Q*, Weaver SJ*, Mock J-Y, Jensen GJ

Molecular architecture, polar targeting and biogenesis of the Legionella Dot/Icm T4SS

March 16, 2023 12:30 PM
Nature Microbiology

Ghosal D*, Jeong KC*, Chang Y-W, Gyore J, Teng L, Gardner A, Vogel JP+, Jensen GJ+

ETDB-Caltech: a blockchain-based distributed public database for electron tomography

March 16, 2023 12:29 PM
PLOS One

Ortega DR, Oikonomou CM, Ding HJ, Rees-Lee P, Alexandria, Jensen GJ

Electron cryotomography of bacterial secretion systems

March 16, 2023 12:28 PM
Microbiology Spectrum

Oikonomou CM, Jensen GJ

Simulations suggest a constrictive force is required for Gram-negative bacterial cell division

March 16, 2023 12:28 PM
Nature Communications

Nguyen LT, Oikonomou CM, Ding HJ, Kaplan M, Yao Q, Chang Y-W, Beeby M, Jensen GJ

De novo structural pattern mining in cellular electron cryotomograms

March 16, 2023 12:27 PM
Structure

Xu M, Singla J, Tocheva EI, Chang Y-W, Stevens RC, Jensen GJ, Alber F

In situ imaging and structure determination of bacterial toxin delivery systems using electron cryotomography

March 16, 2023 12:25 PM
Methods in Molecular Biology

Ghosal D*, Kaplan M*, Chang Y-W, Jensen GJ

FGF21 trafficking in intact human cells revealed by cryo-electron tomography with gold nanoparticles

March 16, 2023 12:25 PM
eLife

Azubel M, Carter SD, Weiszmann J, Zhang J, Jensen GJ, Li Y, Kornberg RD

Collection of continuous rotation MicroED data from ion beam-milled crystals of any size

March 16, 2023 12:24 PM
Structure

Martynowycz MW, Zhao W, Hattne J, Jensen GJ, Gonen T

The presence and absence of periplasmic rings in bacterial flagellar motors correlates with stator type

March 16, 2023 12:23 PM
eLife

Kaplan M, Ghosal D, Subramanian P, Oikonomou CM, Kjaer A, Pirbadian S, Ortega DR, Briegel A, El-Naggar MY, Jensen GJ

Rapid tilt-series acquisition for electron cryotomography

March 16, 2023 12:23 PM
Journal of Structural Biology

Chreifi G*, Chen S*, Metskas LA, Kaplan M, Jensen GJ

2018

Programmed secretion arrest and receptor-triggered toxin export during antibacterial contact-dependent growth inhibition

March 16, 2023 12:20 PM
Cell

Ruhe ZC*, Subramanian P*, Song K*, Nguyen JY, Stevens TA, Low DA, Jensen GJ+, Hayes CS+

Three-dimensional analysis of mitochondrial crista ultrastructure in a patient with Leigh Syndrome by in situ cryoelectron tomography

March 16, 2023 12:17 PM
iScience

Siegmund SE, Grassucci R, Carter SD, Barca E, Farino ZJ, Juanola-Falgarona M, Zhang P, Tanji K, Hirano M, Schon EA, Frank J, Freyberg Z

Selective permeability of carboxysome shell pores to anionic molecules

March 15, 2023 02:28 PM
Journal of Physical Chemistry B

Mahinthichaichan P*, Morris DM*, Wang Y, Jensen GJ+, Tajkhorshid E+

Nutrient transport suggests an evolutionary basis for charged archaeal surface layer proteins

March 15, 2023 02:27 PM
ISME Journal

Li P-N, Herrmann J, Tolar BB, Poitevin F, Ramdasi R, Bargar JR, Stahl DA, Jensen GJ, Francis CA+, Wakatsuki S+, van den Bedem H+

Coarse-grained simulations of actomyosin rings point to a nodeless model involving both unipolar and bipolar myosins

March 15, 2023 02:26 PM
Molecular Biology of the Cell

Nguyen LT, Swulius MT, Aich S, Mishra M, Jensen GJ

In vivo structures of the Helicobacter pylori cag type IV secretion system

March 15, 2023 02:25 PM
Cell Reports

Chang Y-W*, Shaffer CL*, Rettberg LA, Ghosal D, Jensen GJ

Ultrastructure of Shewanella oneidensis MR-1 nanowires revealed by electron cryotomography

March 15, 2023 02:25 PM
PNAS

Subramanian P*, Pirbadian S*, El-Naggar MY+, Jensen GJ+

Electron cryotomography of Mycoplasma pneumoniae mutants correlates terminal organelle architectural features and function

March 15, 2023 02:23 PM
Molecular Microbiology

Krause DC+, Chen S, Shi J, Jensen A, Sheppard ES, Jensen GJ

Structure of the fission yeast actomyosin ring during constriction

March 15, 2023 02:23 PM
PNAS

Swulius MT*, Nguyen LT*, Ladinsky MS, Ortega DR, Aich S, Mishra M, Jensen GJ

2017

Comment: The development of cryo-EM and how it has advanced microbiology

March 15, 2023 01:44 PM
Nature Microbiology

Oikonomou CM, Jensen GJ

Assigning chemoreceptors to chemosensory pathways in Pseudomonas aeruginosa

March 15, 2023 01:44 PM
PNAS

Ortega DR*, Fleetwood AD*, Krell T, Harwood CS, Jensen GJ, Zhulin IB

Distinguishing signal from autofluorescence in cryogenic correlated light and electron microscopy of mammalian cells

March 15, 2023 01:43 PM
Journal of Structural Biology

Carter SD*, Mageswaran SK*, Farino ZJ, Mamede JI, Oikonomou CM, Hope TJ, Freyberg Z+, Jensen GJ+

Nitrosopumilus maritimus gen. nov., sp. nov., Nitrosopumilus cobalaminigenes sp. nov., Nitrosopumilus oxyclinae sp. nov., and Nitrosopumilus ureiphilus sp. nov., four marine ammonia-oxidizing archaea of the phylum Thaumarchaeota

March 15, 2023 01:42 PM
International Journal of Systematic & Evolutionary Microbiology

Qin W, Heal KR, Ramdasi R, Kobelt JN, Martens-Habbena W, Bertagnolli AD, Amin SA, Walker CB, Urakawa H, Könneke M, Devol AH, Moffett JW, Armbrust EV, Jensen GJ, Ingalls AE, Stahl DA

FtsEX-mediated regulation of the final stages of cell division reveals morphogenetic plasticity in Caulobacter crescentus

March 15, 2023 01:42 PM
PLoS Genetics

Meier EL, Daitch AK, Yao Q, Bhargava A, Jensen GJ, Goley ED

LytM factors affect the recruitment of autolysins to the cell division site in Caulobacter crescentus

March 15, 2023 01:37 PM
Molecular Microbiology

Zielińska A*, Billini M*, Möll A, Kremer K, Briegel A, Martinez AI, Jensen GJ, Thanbichler M

Best practices for managing large cryoEM facilities

March 15, 2023 01:36 PM
Journal of Structural Biology

Alewijnse B, Ashton AW, Chambers MG, Chen S, Cheng A, Ebrahim M, Eng E, Hagen WJH, Koster AJ, Lopez CS, Lukoyanova N, Ortega J, Renault L, Reyntjens S, Rice WJ, Scapin G, Schrijver R, Siebert A, Stagg SM, Grum-Tokars V, Wright ER, Wu S, Yu Z, Zhou ZH, Carragher B, Potter CS

Photon-induced near-field electron microscopy (PINEM) of eukaryotic cells

March 15, 2023 01:35 PM
Angewandte Chemie International Edition

Kaplan M, Yoo BK, Tang J+, Karam T, Liao B, Majumdar B, Baltimore D, Jensen G+, Zewail A

Morphology of the archaellar motor and associated cytoplasmic cone in Thermococcus kodakaraensis

March 15, 2023 01:34 PM
EMBO Reports

Briegel A, Oikonomou CM, Chang Y-W, Kjaer A, Huang AN, Kim KW, Ghosal D, Nguyen HH, Kenny D, Ogorzalek Loo RR, Gunsalus RP, Jensen GJ

Polar delivery of Legionella type IV secretion system substrates is essential for virulence

March 15, 2023 01:33 PM
PNAS

Jeong KC, Ghosal D, Chang Y-W, Jensen GJ, Vogel JP

Uncharacterized bacterial structures revealed by electron cryotomography

March 15, 2023 01:33 PM
Journal of Bacteriology

Dobro MJ, Oikonomou CM, Piper A, Cohen J, Guo K, Jensen T, Tadayon J, Donermeyer J, Park Y, Solis BA, Kjaer A, Jewett AI, McDowall AW, Chen S, Chang Y-W, Shi J, Subramanian P, Iancu CV, Li Z, Briegel A, Tocheva EI, Pilhofer M, Jensen GJ

The Structure, Function and Roles of the Archaeal ESCRT Apparatus

March 15, 2023 01:32 PM
Subcellular Biochemistry

Samson RY, Dobro MJ, Jensen GJ, Bell SD

In vivo structures of an intact type VI secretion system revealed by electron cryotomography

March 15, 2023 01:30 PM
EMBO Reports

Chang Y-W, Rettberg LA, Ortega DR, Jensen GJ

Short FtsZ filaments can drive asymmetric cell envelope constriction at the onset of bacterial cytokinesis

March 14, 2023 02:29 PM
The EMBO Journal

Yao Q, Jewett AI, Chang Y-W, Oikonomou CM, Beeby M, Iancu CV, Briegel A, Ghosal D, Jensen GJ

Cellular Electron Cryotomography: Toward Structural Biology In Situ

March 14, 2023 02:28 PM
Annual Review of Biochemistry

Oikonomou CM, Jensen GJ

Giant viruses with an expanded complement of translation system components

March 14, 2023 02:27 PM
Science

Schulz F+, Yutin N, Ivanova NN, Ortega DR, Lee TK, Vierheilig J, Daims H, Horn M, Wagner M, Jensen GJ, Kyrpides NC, Koonin EV+, Woyke T+

Structure of the Legionella Dot/Icm type IV secretion system in situ by electron cryotomography

March 14, 2023 02:26 PM
EMBO Reports

Ghosal D, Chang Y-W, Jeong KC, Vogel JP, Jensen GJ

Polyphosphate granule biogenesis is temporally and functionally tied to cell cycle exit during starvation in Pseudomonas aeruginosa

March 14, 2023 02:25 PM
PNAS

Racki LR, Tocheva EI, Dieterle MG, Sullivan MC, Jensen GJ, Newman DK

Architecture of the Vibrio cholerae toxin-coregulated pilus machine revealed by electron cryotomography

March 14, 2023 02:24 PM
Nature Microbiology

Chang Y-W, Kjaer A, Ortega DR, Kovacikova G, Sutherland JA, Rettberg LA, Taylor RK, Jensen GJ

2016

Dynamics of the peptidoglycan biosynthetic machinery in the stalked budding bacterium Hyphomonas neptunium

March 14, 2023 02:09 PM
Molecular Microbiology

Cserti E, Rosskopf S, Chang Y-W, Eisheuer S, Selter L, Shi J, Regh C, Koert U, Jensen GJ, Thanbichler M

Electron cryotomography

March 14, 2023 02:08 PM
Methods in Microbiology

Oikonomou CM, Swulius MT, Briegel A, Beeby M, Yao Q, Chang Y-W, Jensen GJ

Cryo-EM structure of a CD4-bound open HIV-1 envelope trimer reveals structural rearrangements of the gp120 V1V2 loop

March 14, 2023 02:07 PM
PNAS

Wang H, Cohen AA, Galimidi RP, Gristick HB, Jensen GJ, Bjorkman PJ

Starvation and recovery in the deep-sea methanotroph Methyloprofundus sedimenti

March 14, 2023 02:07 PM
Molecular Microbiology

Tavormina PL+, Kellermann MY, Antony CP, Tocheva E, Dalleska N, Jensen AJ, Valentine DL, Hinrichs KU, Jensen G, Dubilier N, Orphan VJ

Chemotaxis cluster 1 proteins form cytoplasmic arrays in Vibrio cholerae and are stabilized by a double signaling domain receptor DosM

March 14, 2023 02:06 PM
PNAS

Briegel A+, Ortega DR, Mann P, Kjaer A, Ringgaard S, Jensen GJ+

Sporulation, bacterial cell envelopes, and the origin of life

March 14, 2023 02:05 PM
Nature Reviews Microbiology

Tocheva EI+, Ortega DR, Jensen GJ+

Coarse-Grained Molecular Dynamics Simulations of the Bacterial Cell Wall

March 14, 2023 02:04 PM
Methods in Molecular Biology

Nguyen LT, Gumbart JC, Jensen GJ

Primate TRIM5 proteins form hexagonal nets on HIV-1 capsids

March 14, 2023 02:03 PM
eLife

Li YL*, Chandrasekaran V*, Carter SD, Woodward CL, Christensen DE, Dryden KA, Pornillos O, Yeager M, Ganser-Pornillos BK*, Jensen GJ*, Sundquist WI*

Coarse-grained simulation reveals key features of HIV-1 capsid self-assembly

March 14, 2023 02:02 PM
Nature Communications

Grime JMA, Dama JF, Ganser-Pornillos BK, Woodward CL, Jensen GJ, Yeager MJ, Voth GA

Phylogenomic analysis of Candidatus ‘Izimaplasma’ species: free-living representatives from a Tenericutes clade found in methane seeps

March 14, 2023 02:01 PM
ISME Journal

Skennerton CT+, Haroon MF, Briegel A, Shi J, Jensen GJ, Tyson GW, Orphan VJ

Diverse high-torque bacterial flagellar motors assemble wider stator rings using a conserved protein scaffold

March 14, 2023 02:00 PM
PNAS

Beeby M, Ribardo DA, Brennan CA, Ruby EG, Jensen GJ, Hendrixson DR

Architecture of the type IVa pilus machine

March 14, 2023 02:00 PM
Science

Chang Y-W, Rettberg LA, Treuner-Lange A, Iwasa J, Sogaard-Andersen L, Jensen GJ

A new view into prokaryotic cell biology from electron cryotomography

March 14, 2023 01:59 PM
Nature Reviews Microbiology

Oikonomou CM, Chang Y-W, Jensen GJ

Dynamic Remodeling of the Magnetosome Membrane Is Triggered by the Initiation of Biomineralization

March 14, 2023 01:58 PM
MBio

Cornejo E, Subramanian P, Li Z, Jensen GJ, Komeili A

2015

The Caltech Tomography Database and Automatic Processing Pipeline

March 14, 2023 01:56 PM
Journal of Structural Biology

Ding HJ, Oikonomou, CM, Jensen GJ

Direct visualization of vaults within intact cells by electron cryo-tomography

March 14, 2023 01:55 PM
Cellular and Molecular Life Sciences

Woodward CL, Mendonça LM, Jensen GJ

Structural conservation of chemotaxis machinery across Archaea and Bacteria

March 14, 2023 01:54 PM
Environmental Microbiology Reports

Briegel A, Ortega DR, Huang AN, Oikonomou CM, Gunsalus RP, Jensen GJ

Electron cryotomography studies of maturing HIV-1 particles reveal the assembly pathway of the viral core

March 14, 2023 01:49 PM
Journal of Virology

Woodward CL, Cheng SN, Jensen GJ

Streptomyces: a screening tool for bacterial cell division inhibitors

March 14, 2023 01:48 PM
Journal of Biomolecular Screening

Jani C, Tocheva EI, McAuley S, Craney A, Jensen GJ, Nodwell J

2014

Ultrastructure and complex polar architecture of the human pathogen Campylobacter jejuni

March 14, 2023 01:25 PM
Microbiology Open

Müller A, Beeby M, McDowall AW, Chow J, Jensen GJ, Clemons WM Jr.

Correlated cryogenic photoactivated localization microscopy and cryo-electron tomography

March 14, 2023 01:24 PM
Nature Methods

Chang YW, Chen S, Tocheva EI, Treuner-Lange A, Löbach S, Søgaard-Andersen L, Jensen GJ

Structure of bacterial cytoplasmic chemoreceptor arrays and implications for chemotactic signaling

March 14, 2023 01:23 PM
eLife

Briegel A, Ladinsky MS, Oikonomou C, Jones CW, Harris MJ, Fowler DJ, Chang YW, Thompson LK, Armitage JP, Jensen GJ

Escherichia coli peptidoglycan structure and mechanics as predicted by atomic-scale simulations

March 14, 2023 01:23 PM
PLOS Computational Biology

Gumbart JC+, Beeby M, Jensen GJ, Roux B+

New insights into bacterial chemoreceptor array structure and assembly from electron cryotomography

March 14, 2023 01:22 PM
Biochemistry

Briegel A, Wong ML, Hodges HL, Oikonomou CM, Piasta KN, Harris MJ, Fowler DJ, Thompson LK, Falke JJ, Kiessling LL, Jensen GJ

Structure and expression of propanediol utilization microcompartments in Acetonema longum

March 14, 2023 01:21 PM
Journal of Bacteriology

Tocheva EI, Matson EG, Cheng SN, Chen WG, Leadbetter JR, Jensen GJ

Marine tubeworm metamorphosis induced by arrays of bacterial phage tail-like structures

March 14, 2023 01:21 PM
Science

Shikuma NJ*, Pilhofer M*, Weiss GL, Hadfield MG+, Jensen GJ+, Newman DK+

2013

Discovery of chlamydial peptidoglycan reveals bacteria with murein sacculi but without FtsZ

March 14, 2023 01:20 PM
Nature Communications

Pilhofer M*, Aistleitner K*, Biboy J, Gray J, Kuru E, Hall E, Brun YV, VanNieuwenhze MS, Vollmer W, Horn M+, Jensen GJ+

Architecture and host interface of environmental chlamydiae revealed by electron cryotomography

March 14, 2023 01:18 PM
Environmental Microbiology

Pilhofer M*, Aistleitner K*, Ladinsky MS, König L, Horn M+, Jensen GJ+

Chromatin in a marine picoeukaryote is a disordered assemblage of nucleosomes

March 14, 2023 01:18 PM
Chromosoma

Gan L+, Ladinsky MS, Jensen GJ

Polyphosphate storage during sporulation in the gram-negative bacterium Acetonema longum

March 14, 2023 01:17 PM
Journal of Bacteriology

Tocheva EI, Dekas AE, McGlynn SE, Morris D, Orphan VJ, Jensen GJ

The mobility of two kinase domains in the Escherichia coli chemoreceptor array varies with signalling state

March 14, 2023 01:16 PM
Molecular Microbiology

Briegel A, Ames P, Gumbart JC, Oikonomou CM, Parkinson JS, Jensen GJ

Electron cryotomography of ESCRT assemblies and dividing Sulfolobus cells suggests that spiraling filaments are involved in membrane scission

March 14, 2023 01:15 PM
Molecular Biology of the Cell

Dobro MJ, Samson RY, Yu Z, McCullough J, Ding HJ, Chong PL, Bell SD, Jensen GJ

The challenge of determining handedness in electron tomography and the use of DNA origami gold nanoparticle helices as molecular standards

March 14, 2023 01:14 PM
Journal of Structural Biology

Briegel A*, Pilhofer M*, Mastronarde DN, Jensen GJ

Architecture and assembly of the Gram-positive cell wall

March 14, 2023 01:13 PM
Molecular Microbiology

Beeby M, Gumbart JC, Roux B, Jensen GJ

Peptidoglycan transformations during Bacillus subtilis sporulation

March 14, 2023 01:12 PM
Molecular Microbiology

Tocheva EI, López-Garrido J, Hughes HV, Fredlund J, Kuru E, Vannieuwenhze MS, Brun YV, Pogliano K, Jensen GJ

Architecture of the major component of the type III secretion system export apparatus

March 14, 2023 01:11 PM
Nature Structural and Molecular Biology

Abrusci P, Vergara-Irigaray M, Johnson S, Beeby MD, Hendrixson DR, Roversi P, Friede ME, Deane JE, Jensen GJ, Tang CM, Lea SM

Unclosed HIV-1 capsids suggest a curled sheet model of assembly

March 14, 2023 01:10 PM
Journal of Molecular Biology

Yu Z*, Dobro MJ*, Woodward CL, Levandovsky A, Danielson CM, Sandrin V, Shi J, Aiken C, Zandi R, Hope TJ, Jensen GJ

2012

Data management challenges in three-dimensional EM

March 14, 2023 01:00 PM
Nature Structural and Molecular Biology

Patwardhan A, Carazo JM, Carragher B, Henderson R, Heymann JB, Hill E, Jensen GJ, Lagerstedt I, Lawson CL, Ludtke SJ, Mostronarde D, Moore WJ, Roseman A, Rosenthal P, Sorzano CO, Sanz-Garcia E, Scheres SH, Subramaniam S, Westbrook J, Winn M, Swedlow JR+, Kleywegt GJ+

General protein diffusion barriers create compartments within bacterial cells

March 14, 2023 12:59 PM
Cell

Schlimpert S*, Klein EA*, Briegel A, Hughes V, Kahnt J, Bolte K, Maier UG, Brun YV, Jensen GJ, Gitai Z, Thanbichler M

The bacterial cytoskeleton: more than twisted filaments

March 14, 2023 12:59 PM
Current Opinion in Cell Biology

Pilhofer M, Jensen GJ

A multidomain hub anchors the chromosome segregation and chemotactic machinery to the bacterial pole

March 14, 2023 12:58 PM
Genes and Development

Yamaichi Y*, Bruckner R*, Ringgaard S, Möll A, Cameron DE, Briegel A, Jensen GJ, Davis BM, Waldor MK

Type VI secretion requires a dynamic contractile phage tail-like structure

March 14, 2023 12:52 PM
Nature

Basler M*, Pilhofer M*, Henderson GP, Jensen GJ+, Mekalanos JJ+

Bacterial chemoreceptor arrays are hexagonally packed trimers of receptor dimers networked by rings of kinase and coupling proteins

March 14, 2023 12:48 PM
PNAS

Briegel A*, Li X*, Bilwes AM, Hughes KT, Jensen GJ+, Crane BR+

Growth and localization of polyhydroxybutyrate granules in Ralstonia eutropha

March 14, 2023 12:47 PM
Journal of Bacteriology

Beeby M, Cho M, Stubbe J, Jensen GJ

Electron Tomography of Cells

March 14, 2023 12:43 PM
Quarterly Reviews of Biophysics

Gan L, Jensen GJ

2011

Microtubules in bacteria: Ancient tubulins build a five-protofilament homolog of the eukaryotic cytoskeleton

March 14, 2023 12:41 PM
PLoS Biology

Pilhofer M+, Ladinsky MS, McDowall AW, Petroni G, Jensen GJ+

Activated chemoreceptor arrays remain intact and hexagonally packed

March 14, 2023 12:40 PM
Molecular Microbiology

Briegel A, Beeby M, Thanbichler M, Jensen GJ

Organization of the smallest eukaryotic spindle

March 14, 2023 12:40 PM
Current Biology

Gan L, Ladinsky MS, Jensen GJ

Peptidoglycan remodeling and conversion of an inner membrane into an outer membrane during sporulation

March 14, 2023 12:39 PM
Cell

Tocheva EI, Matson EG, Morris DM, Moussavi F, Leadbetter JR, Jensen GJ

Nanopods: a new bacterial structure and mechanism for deployment of outer membrane vesicles

March 14, 2023 12:39 PM
PLoS

Shetty A, Chen S, Tocheva EI, Jensen GJ, Hickey WJ

Structural diversity of bacterial flagellar motors

March 14, 2023 12:36 PM
The EMBO Journal

Chen S*, Beeby M*, Murphy GE, Leadbetter JR, Hendrixson DR, Briegel A, Li Z, Shi J, Tocheva EI, Müller A, Dobro MJ, Jensen GJ

Alternative mechanism for bacteriophage adsorption to the motile bacterium Caulobacter crescentus

March 14, 2023 12:36 PM
PNAS

Guerrero-Ferreira RC, Viollier PH, Ely B, Poindexter JS, Georgieva M, Jensen GJ, Wright ER

Long helical filaments are not seen encircling cells in electron cryotomograms of rod-shaped bacteria

March 14, 2023 12:35 PM
Biochemical and Biophysical Research Communications

Swulius MT, Chen S, Jane Ding H, Li Z, Briegel A, Pilhofer M, Tocheva EI, Lybarger SR, Johnson TL, Sandkvist M, Jensen GJ

2010

In vivo assembly of an archaeal virus studied with whole-cell electron cryotomography

March 14, 2023 12:25 PM
Structure

Fu CY, Wang K, Gan L, Lanman J, Khayat R, Young MJ, Jensen GJ, Doerschuk PC, Johnson JE

Correcting for the Ewald sphere in high-resolution single-particle reconstructions

March 14, 2023 12:25 PM
Methods in Enzymology

Leong PA, Yu X, Zhou ZH, Jensen GJ

Cryo-EM, Part B: 3-D Reconstruction

March 14, 2023 12:23 PM
Methods in Enzymology

Jensen GJ

Correlated light and electron cryo-microscopy

March 14, 2023 12:21 PM
Methods in Enzymology

Briegel A, Chen S, Koster AJ, Plitzko JM, Schwartz CL, Jensen GJ

Plunge freezing for electron cryomicroscopy

March 14, 2023 12:21 PM
Methods in Enzymology

Dobro MJ, Melanson LA, Jensen GJ, McDowall AW

Bacterial TEM: new insights from cryo-microscopy

March 14, 2023 12:19 PM
Methods in Cell Biology

Pilhofer M, Ladinsky MS, McDowall AW, Jensen GJ

The metabolic enzyme CTP synthase forms cytoskeletal filaments

March 14, 2023 12:18 PM
Nature Cell Biology

Ingerson-Mahar M, Briegel A, Werner JN, Jensen GJ, Gitai Z

Electron cryotomography

March 14, 2023 12:18 PM
Cold Spring Harbor Perspectives in Biology

Tocheva EI, Li Z, Jensen GJ

DipM, a new factor required for peptidoglycan remodelling during cell division in Caulobacter crescentus

March 14, 2023 12:17 PM
Molecular Microbiology

Möll A, Schlimpert S, Briegel A, Jensen GJ, Thanbichler M

Electron cryotomography of bacterial cells

March 14, 2023 12:15 PM
JoVE

Chen S, McDowall A, Dobro MJ, Briegel A, Ladinsky M, Shi J, Tocheva EI, Beeby M, Pilhofer M, Ding HJ, Li Z, Gan L, Morris DM, Jensen GJ

Mutations in the Lipopolysaccharide biosynthesis pathway interfere with crescentin-mediated cell curvature in Caulobacter crescentus

March 09, 2023 02:30 PM
Journal of Bacteriology

Cabeen MT*, Murolo MA*, Briegel A, Bui NK, Vollmer W, Ausmees N, Jensen GJ, Jacobs-Wagner C

Bactofilins, a ubiquitous class of cytoskeletal proteins mediating polar localization of a cell wall synthase in Caulobacter crescentus

March 09, 2023 02:29 PM
The EMBO Journal

Kühn J, Briegel A, Mörschel E, Kahnt J, Leser K, Wick S, Jensen GJ, Thanbichler M

Organization, structure, and assembly of alpha-carboxysomes determined by electron cryotomography of intact cells

March 09, 2023 02:28 PM
Journal of Molecular Biology

Iancu CV*, Morris DM*, Dou Z, Heinhorst S, Cannon GC, Jensen GJ

2009

Universal architecture of bacterial chemoreceptor arrays

March 09, 2023 02:24 PM
PNAS

Briegel A, Ortega DR, Tocheva EI, Wuichet K, Li Z, Chen S, Müller A, Iancu CV, Murphy GE, Dobro MJ, Zhulin IB, Jensen GJ

Nanogold as a specific marker for electron cryotomography

March 09, 2023 02:23 PM
Microscopy and Microanalysis

He Y, Jensen GJ, Bjorkman PJ

Electron cryotomography: a new view into microbial ultrastructure

March 09, 2023 02:22 PM
Current Opinion in Microbiology

Li Z, Jensen GJ

Fully automated, sequential tilt-series acquisition with Leginon

March 09, 2023 02:21 PM
Journal of Structural Biology

Suloway C, Shi J, Cheng A, Pulokas J, Carragher B, Potter CS, Zheng SQ, Agard DA, Jensen GJ

Cell biology. Protein filaments caught in the act

March 09, 2023 02:19 PM
Science

Jensen GJ

2008

Molecular organization of Gram-negative peptidoglycan

March 09, 2023 02:02 PM
PNAS

Gan L, Chen S, Jensen GJ

FcRn-mediated antibody transport across epithelial cells revealed by electron tomography

March 09, 2023 02:01 PM
Nature

He W, Ladinsky MS, Huey-Tubman KE, Jensen GJ, McIntosh JR, Björkman PJ

A self-associating protein critical for chromosome attachment, division, and polar organization in caulobacter

March 09, 2023 02:01 PM
Cell

Ebersbach G, Briegel A, Jensen GJ, Jacobs-Wagner C

An improved cryogen for plunge freezing

March 09, 2023 02:00 PM
Microscopy and Microanalysis

Tivol WF+, Briegel A, Jensen GJ

Radiation dose reduction and image enhancement in biological imaging through equally-sloped tomography

March 09, 2023 01:56 PM
Journal of Structural Biology

Lee E*, Fahimian BP*, Iancu CV, Suloway C, Murphy GE, Wright ER, Castaño-Díez D, Jensen GJ, Miao J

Location and architecture of the Caulobacter crescentus chemoreceptor array

March 09, 2023 01:55 PM
Molecular Microbiology

Briegel A, Ding HJ, Li Z, Werner J, Gitai Z, Dias DP, Jensen RB, Jensen GJ

Toward a biomechanical understanding of whole bacterial cells

March 09, 2023 01:54 PM
Annual Review of Biochemistry

Morris DM, Jensen GJ

Cryo-EM structure of dodecameric Vps4p and its 2:1 complex with Vta1p

March 09, 2023 01:52 PM
Journal of Molecular Biology

Yu Z, Gonciarz MD, Sundquist WI, Hill CP, Jensen GJ

Novel ultrastructures of Treponema primitia and their implications for motility

March 08, 2023 02:20 PM
Molecular Microbiology

Murphy GE, Matson EG, Leadbetter JR, Berg HC, Jensen GJ

2007

Tetrasphaera remsis sp. nov., isolated from the Regenerative Enclosed Life Support Module Simulator (REMS) air system

March 08, 2023 02:17 PM
International Journal of Systematic and Evolutionary Microbiology

Osman S, Moissl C, Hosoya N, Briegel A, Mayliraj S, Satomi M, Venkateswaran K

Electron cryotomography

March 08, 2023 02:17 PM
Biotechniques

Murphy GE, Jensen GJ

The structure of FtsZ filaments in vivo suggests a force-generating role in cell division

March 08, 2023 02:16 PM
The EMBO Journal

Li Z, Trimble MJ, Brun YV, Jensen GJ

3-D ultrastructure of O. tauri: electron cryotomography of an entire eukaryotic cell

March 08, 2023 02:15 PM
PLoS One

Henderson GP, Gan L, Jensen GJ

The structure of isolated Synechococcus strain WH8102 carboxysomes as revealed by electron cryotomography

March 08, 2023 02:13 PM
Journal of Molecular Biology

Iancu CV, Ding HJ, Morris DM, Dias DP, Gonzales AD, Martino A, Jensen GJ

How electron cryotomography is opening a new window onto prokaryotic ultrastructure

March 08, 2023 02:12 PM
Current Opinion in Structural Biology

Jensen GJ, Briegel A

Electron cryotomography of immature HIV-1 virions reveals the structure of the CA and SP1 Gag shells

March 08, 2023 02:11 PM
The EMBO Journal

Wright ER*, Schooler JB*, Ding HJ, Kieffer C, Fillmore C, Sundquist WI, Jensen GJ

Haloquadratum walsbyi gen. nov., sp. nov., the square haloarchaeon of Walsby, isolated from saltern crystallizers in Australia and Spain

March 08, 2023 02:10 PM
Int'l Journal of Systematic & Evolutionary Microbiology

Burns DG, Janssen PH, Itoh T, Kamekura M, Li Z, Jensen G, Rodriguez-Valera F, Bolhuis H, Dyall-Smith ML

2006

In situ structure of the complete Treponema primitia flagellar motor

March 08, 2023 01:30 PM
Nature

Murphy GE, Leadbetter JR, Jensen GJ

Multiple large filament bundles observed in Caulobacter crescentus by electron cryotomography

March 08, 2023 01:28 PM
Molecular Microbiology

Briegel A, Dias DP, Li Z, Jensen RB, Frangakis AS, Jensen GJ

Electron cryotomography sample preparation using the Vitrobot

March 08, 2023 01:28 PM
Nature Protobols

Iancu CV, Tivol WF, Schooler JB, Dias DP, Henderson GP, Murphy GE, Wright ER, Li Z, Yu Z, Briegel A, Gan L, He Y, Jensen GJ

Rigid, specific, and discrete gold nanoparticle/antibody conjugates

March 08, 2023 01:25 PM
Journal of the American Chemical Society

Ackerson CJ, Jadzinsky PD, Jensen GJ, Kornberg RD

Observations on the behavior of vitreous ice at approximately 82 and approximately 12 K

March 08, 2023 01:24 PM
Journal of Structural Biology

Wright ER*, Iancu CV*, Tivol WF, Jensen GJ

A comparison of liquid nitrogen and liquid helium as cryogens for electron cryotomography

March 08, 2023 01:14 PM
Journal of Structural Biology

Iancu CV, Wright ER, Heymann JB, Jensen GJ

Magnetosomes are cell membrane invaginations organized by the actin-like protein MamK

March 08, 2023 01:13 PM
Science

Komeili A*+, Li Z*, Newman DK, Jensen GJ

2005

A “flip-flop” rotation stage for routine dual-axis electron cryotomography

March 08, 2023 01:10 PM
Journal of Structural Biology

Iancu CV*, Wright ER*, Benjamin J, Tivol WF, Dias DP, Murphy GE, Morrison RC, Heymann JB, Jensen GJ

Three-dimensional structure of HIV-1 virus-like particles by electron cryotomography

March 08, 2023 12:59 PM
Journal of Molecular Biology

Benjamin J, Ganser-Pornillos BK, Tivol WF, Sundquist WI, Jensen GJ